Cloud point method applied to the Apolipoprotein A-I extraction from human plasma and its identification by tandem mass spectrometry

DOI: 10.5584/jiomics.v1i1.27

Authors

  • Marco Aurélio Zezzi Arruda Group of Spectrometry, Sample Preparation and Mechanization-GEPAM, Department of Analytical Chemistry, Institute of Chemistry, P.O. Box 6154, University of Campinas – UNICAMP, 13083-970, Campinas, SP, Brazil. https://orcid.org/0000-0002-7058-3390
  • Aline Soriano Lopes National Institute of Science and Technology for Bioanalyitics, Institute of Chemistry, University of Campinas – UNICAMP, PO Box 6154, 13083-970, Campinas, SP, Brazil
  • Marcelo Anselmo Oseas da Silva National Institute of Science and Technology for Bioanalyitics, Institute of Chemistry, University of Campinas – UNICAMP, PO Box 6154, 13083-970, Campinas, SP, Brazil
  • Fábio Cesar Gozzo National Institute of Science and Technology for Bioanalyitics, Institute of Chemistry, University of Campinas – UNICAMP, PO Box 6154, 13083-970, Campinas, SP, Brazil

Abstract

This work describes the extraction of the apolipoprotein A-I (apoA-I) from human plasma using the cloud point extraction (CPE). The CPE was carried out with a nonionic surfactant (5 % w/v Triton® X-114), and the presence of a salting-out effect (10 % w/v NaCl) promoted biocompatible separation conditions at room temperature and pH 6.8. The apoA-I present in the surfactant-rich phase was identified by tandem mass spectrometry after two-dimensional gel electrophoresis.

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Published

2021-02-14

Issue

Vol. 1 No. 1 (2011): Vol. 1 No. 1 (2011)

Section

Articles

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Copyright (c) 2011 Creative Common Licenses

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This work is licensed under a Creative Commons Attribution 3.0 Unported License.