Revisiting Protocols for the NMR Analysis of Bacterial Metabolomes

DOI: 10.5584/jiomics.v3i2.139

Authors

  • Steven Halouska Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304
  • Bo Zhang Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304
  • Rosmarie Gaupp School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905
  • Emily Snell Department of Chemistry, University of Nebraska-Lincoln, Lincoln, NE 68588-0304
  • Robert J. Fenton School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905
  • Raul G. Barletta School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-090
  • Greg A. Somerville School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-090
  • Robert Powers School of Veterinary Medicine and Biomedical Sciences, University of Nebraska-Lincoln, Lincoln, NE 68588-0905

Abstract

Over the past decade, metabolomics has emerged as an important technique for systems biology. Measuring all the metabolites in a biological system provides an invaluable source of information to explore various cellular processes, and to investigate the impact of environmental factors and genetic modifications. Nuclear magnetic resonance (NMR) spectroscopy is an important method routinely employed in metabolomics. NMR provides comprehensive structural and quantitative information useful for metabolomics fingerprinting, chemometric analysis, metabolite identification and metabolic pathway construction. A successful metabolomics study relies on proper experimental protocols for the collection, handling, processing and analysis of metabolomics data. Critically, these protocols should eliminate or avoid biologically-irrelevant changes to the metabolome. We provide a comprehensive description of our NMR-based metabolomics procedures optimized for the analysis of bacterial metabolomes. The technical details described within this manuscript should provide a useful guide to reliably apply our NMR-based metabolomics methodology to systems biology studies.

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Published

2021-02-22

Issue

Vol. 3 No. 2 (2013): Vol 3, No 2 (2013)

Section

Articles